A double-reciprocal graph is a graph where the values on both axes are the reciprocals of the actual data. Double-reciprocal is a synonym for a Woolf-Lineweaver-Burk plot. The plot describes how quickly an enzyme converts its substrate to its output. On the x-axis is the reciprocal of the concentration of the substrate. On the y-axis is the reciprocal of the speed at which the enzyme can consume the substrate concentration. Use Microsoft Excel to graph a double-reciprocal plot.
Type "S," "V," "1/S" and "1/V" into cells A1, B1, C1 and D1, respectively.
Type values for "S," the enzyme concentration, in column A, beginning with cell A2. For example, type the numbers one through 10 into cells A2 through A11.
Type values for "V," the speed of substrate consumption, in column B, beginning with cell B2. For example, type the numbers 11 through 20 into cells B2 through B11.
Type "=1/A2" into cell C2. Copy that cell and paste it in cells C3 through C11.
Type "=1/B2" into cell D2. Copy and paste it into cells D3 through D11 as well.
Select cells C1 through D11.
Click the "Insert" menu. Click "Scatter," Click "Scatter with Smooth Lines and Markers," the top-right graph option. A double-reciprocal plot will appear.